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SmartRTTM Reverse Transcriptase is an engineered MMLV RT that improves the thermostability of the enzyme, reduces RNase H activity and its cDNA synthesis ability. The enzyme also has a terminal transferase activity, where it adds a few extra nucleotides to the end of the synthesized cDNA. Together with a 3’ modified oligo dT primer and a 5’ SMART universal oligo, which contains a terminal complementation to nucleic acids at the 3’ end of the first-strand cDNA, the SmartRTTM reverse transcriptase produces RACE ready full-length cDNA.
First Strand cDNA Synthesis Kit can be used to synthesize the first strand cDNA.
MCLAB's All-In-One 5X Reverse Transcription Mix Kit is optimized to synthesize the first strand cDNA for RT-PCR and RT-qPCR.
Phage RNA polymerases are widely used for in vitro synthesis of RNA transcripts from DNA templates, which have a double-stranded promoter (at least 19 bases) upstream of the sequence to be transcribed.
The RNA Poly(A) Tailing Kit provides a highly pure and active enzyme and other reagents for quickly and easily adding a poly(A) tail to the 3' end of any RNA.
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