RecA Protein, Tth

Description

Description:
RecA Protein (Tth) is a RecA homolog isolated from Thermus thermophilus. It has a ssDNA-dependent ATPase activity at an optimal temperature between 65 to 75ºC.
The extreme thermostability makes Tth RecA ideal for molecular biology applications that require an elevated temperature condition, such as nucleic acid amplification and sequencing.

Source:
Purified from an E. coli strain carrying a plasmid that overexpresses the recA gene from Thermus thermophilus.

Applications:

• Visualization of DNA structures for electron microscopy ⁽¹⁾
• Site-directed mutagenesis through D-loop ^(2,3)
• Screening of DNA libraries using RecA-probe filaments ^(4,5)
• Targeted cleavage of DNA ⁽⁶⁾
• Improvement of PCR specificity and yield ⁽⁷⁾

Storage Buffer:
10 mM Tris-HCl
100 mM KCl
0.1 mM EDTA
1 mM DTT
0.1% Triton X-100
50% Glycerol
pH7.5 @ 25ºC

Unit Definition:
Sold by mass of pure protein as determined by OD₂₈₀.

Recommended Storage Condition: -20°C

Reference:
1. Radding, C. M. (1991) J Biol Chem 266, 5355-8.
2. Wasserman, S. A. & Cozzarelli, N. R. (1985) Proc Natl Acad Sci U S A 82, 1079-83.
3. Biet, E., Maurisse, R., Dutreix, M. & Sun, J. (2001) Biochemistry 40, 1779-86.
4. Shortle, D., Koshland, D., Weinstock, G. M. & Botstein, D. (1980) Proc Natl Acad Sci U S A 77, 5375-9.
5. Rigas, B., Welcher, A. A., Ward, D. C. & Weissman, S. M. (1986) Proc Natl Acad Sci U S A 83, 9591-5.
6. Honigberg, S. M., Rao, B. J. & Radding, C. M. (1986) Proc Natl Acad Sci U S A 83, 9586-90.
7. Koob, M., Burkiewicz, A., Kur, J. & Szybalski, W. (1992) Nucleic Acids Res 20, 5831-6.

Additional information

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