Description
Amphiregulin (AR) is an EGF related growth factor that signals through the EGF/TGF-α receptor, and stimulates growth of keratinocytes, epithelial cells and some fibroblasts. Amphiregulin also inhibits the growth of certain carcinoma cell lines. Synthesized as a transmembrane protein, Amphiregulin’s extracellular domain is proteolytically processed to release the mature protein. There are 6 conserved cysteine residues, which form 3 intramolecular disulfide bonds essential for biological activity. 

Full Name
Amphiregulin

Source
E. coli

Species
Human

Accession #
Q5U026

Molecule Weight
The mature recombinant human AR is a 11 kDa protein containing 98aa (S101-K198).

Purity
> 95 % as determined by SDS-PAGE

Endotoxin
< 0.1 EU per μg of the protein as determined by the LAL method

Activity
Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 5-15 ng/mL.

Storage
-80     Avoid repeated freeze-thaw cycles.

Storage Buffer
PBS