Description
Angiopoietin-1 (Ang-1) is a secreted glycoprotein that plays a critical role in the development and maintenance of the vascular system. It signals through the receptor tyrosine kinase Tie-2 and binds a variety of integrins and the extracellular matrix. Angiopoietin-1 induces the development and branching of blood vessels, promotes vascular endothelial cell motility during angiogenesis, and enhances vascular integrity. It can suppress several VEGF-induced effects on the vasculature. Angiopoietin-1 can protect against pulmonary arterial hypertension, reduce the extent of fibrosis and remodeling in infarcted diabetic myocardium, and enhance tumor progression and metastasis.

Full Name
Angiopoietin-1

Source
E. coli

Species
Human

Accession #
Q5HYA0

Molecule Weight
The mature recombinant human Ang-1 is a 56 kDa protein containing 479aa (S20-F498).

Purity
> 95 % as determined by SDS-PAGE

Endotoxin
< 0.1 EU per μg of the protein as determined by the LAL method

Activity
Measured by its ability to inhibit serum deprivation induced apoptosis in HUVEC human umbilical vein endothelial cells. The ED50 for this effect is 10-40 ng/mL.

Storage
-80     Avoid repeated freeze-thaw cycles.

Storage Buffer
PBS