Description
BMP-3 is a member of the Bone Morphogenetic Protein (BMPs) family, which is important for the development of bone and cartilage. BMPs belong to the TGF-β superfamily of structurally related signaling proteins. BMP proteins are synthesized as large precursor molecules that are cleaved by proteolytic enzymes. The active form of BMPs can consist of a dimer of two identical proteins or a heterodimer of two related bone morphogenetic proteins. BMPs signal through type I and type II receptor tyrosine kinases and signal through SMAD proteins.
BMP-3 is a potent osteoinductive cytokine, capable of inducing bone and cartilage formation in association with an osteoconductive carrier such as collagen and synthetic hydroxyapatite. In addition to its osteogenic activity, BMP-3 appears to play an important role in cardiac morphogenesis, and is expressed in a variety of other tissues, including lung, liver, spleen, prostate, ovary, and small intestine.  

Full Name
Bone morphogenetic protein 3

Source
E. coli

Species
Human

Accession #
P12645.1

Molecule Weight
The mature recombinant human BMP-3 consists of 110 amino acids (363-472) and has a predicted molecular mass of 12.5 kDa.

Purity
> 95 % as determined by SDS-PAGE

Endotoxin
< 0.1 EU per μg of the protein as determined by the LAL method

Activity
Measured by its ability to inhibit BMP-2-induced activity in MC3T3‑E1 mouse preosteoblast cells. 30 µg/mL of hBMP-3 will antagonize hBMP-2 (0.25 µg/mL) induction of alkaline phosphatase in MC3T3E1 cells by >50%.

Storage
-80     Avoid repeated freeze-thaw cycles.

Storage Buffer
PBS