Description
Fibroblast growth factor 5 is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. 
In vitro, rhFGF5 is a mitogen for Balb/3T3 fibroblasts and bovine heart endothelial cells. FGF5 is also a major muscle-derived survival factor for cultured spinal motoneurons. In vivo, FGF5 is assumed to play central roles in both embryology and neurobiology. Developmentally, FGF5 mRNA is originally found in the embryoblast followed by the lateral somatic mesoderm, where it may play a part in angiogenesis, as well as the myotomes cranial to the tail region, where it may delay terminal myoblast differentiation during cell migration.

Full Name
Human Fibroblast Growth Factor 5

Source
E. coli

Species
Human

Accession #
P12034

Molecule Weight
The mature recombinant human FGF-5 consists of 243 amino acids (26-268) and has a predicted molecular mass of 27 kDa.

Purity
> 95 % as determined by SDS-PAGE

Endotoxin
< 0.1 EU per μg of the protein as determined by the LAL method

Activity
Measured in a cell proliferation assay using NR6R­3T3 mouse fibroblast cells. The ED50 for this effect is 2-10 ng/mL in the presence of 1 µg/mL of heparin.

 Storage
-80     Avoid repeated freeze-thaw cycles.

Storage Buffer
PBS