Description
Human HB-EGF (Heparin-Binding EGF-like growth factor) is a 12-16 kDa member of the EGF family of peptide growth factors. Also known as the DTR (diphtheria toxin receptor), it is further classified as a group 2 ErbB ligand based on its ability to activate both the EGF/ErbB1 and ErbB4 receptors.  HB-EGF expressed in bronchial epithelium cells, visceral and vascular smooth muscle, CD4+ T cells, cardiac muscle, glomerular podocytes, keratinocytes and IL-10-secreting regulatory macrophages. Activity associated with ErbB4 binding appears to be limited to non-mitogenic actions, while EGFR binding induces both mitogenic and non-mitogenic activity.

Full Name
Heparin-Binding EGF-like growth factor

Source
E. coli

Species
Human

Accession #
Q99075

Molecule Weight
The mature recombinant human HB-EGF is a 9.7 kDa protein containing 46aa (D63-L148).

Purity
> 95 % as determined by SDS-PAGE

Endotoxin
< 0.1 EU per μg of the protein as determined by the LAL method

Activity
Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.15-0.75 ng/Ml

Storage
-80     Avoid repeated freeze-thaw cycles.

Storage Buffer
PBS