Description
Insulin-like growth factor I, also known as somatomedin C, is the dominant effector of growth hormone IGF-I/IGF-1 induces the proliferation, migration, and differentiation of a wide variety of cell types during development and postnatally. IGF-I/IGF-1 regulates glucose and fatty acid metabolism, steroid hormone activity, and cartilage and bone metabolism. It plays an important role in muscle regeneration and tumor progression. IGF-I/IGF-1 binds IGF-I R, IGF-II R, and the insulin receptor, although its effects are mediated primarily by IGF-I R. IGF-I/IGF-1 association with IGF binding proteins increases its plasma half‑life and modulates its interactions with receptors.

Full Name
Insulin-like Growth Factor 1

Source
E. coli

Species
Human

Accession #
P05019

Molecule Weight
The mature recombinant human IGF-1 is a 7.6 kDa protein containing 70aa (G49-A118).

Purity
> 95 % as determined by SDS-PAGE

Endotoxin
< 0.1 EU per μg of the protein as determined by the LAL method

Activity
Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells. The ED50 for this effect is 0.3-1.5 ng/mL.

Storage
-80     Avoid repeated freeze-thaw cycles.

Storage Buffer
PBS