Recombinant Human Wnt-5a Protein
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The Wnts is a large family of secreted proteins with roles in pattern formation, cell fate decision, axon guidance, and tumor formation. Wnt signaling induces and maintains the transformed phenotype and, in certain embryonic cell lines, supports self-renewal in the absence of significant differentiation.
SKU | OPTIONS | Price | Quantity | ||
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WNT5a-100 | 50 ug (1 mg/ml) | Please inquire | |||
WNT5a-200 | 1 mg (1 mg/ml) | Please inquire | |||
WNT5a-300 | 100 mg (10 mg/ml) | Please inquire | |||
WNT5a-400 | 1 g (10 mg/ml) | Please inquire | |||
WNT5a-OEM | Any size | Please inquire |
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Description
Description
The Wnts is a large family of secreted proteins with roles in pattern formation, cell fate decision, axon guidance, and tumor formation. Wnt signaling induces and maintains the transformed phenotype and, in certain embryonic cell lines, supports self-renewal in the absence of significant differentiation. Elevated levels of Wnt proteins are associated with tumorigenesis and are present in numerous human breast cancers. Wnt family members are highly hydrophobic, and possess 22 to 24 conserved cysteines.
Wnt 5a belongs to the non-canonical class of Wnt proteins which are independent of or inhibit β-catenin signalling. Wnt5a when bound to its receptor triggers an intracellular Ca release which activates PKC and CaMK11 which in turn activates TAK1 MAPkinase-kinase and nemo-like (NLK) kinase. NLK phosphorylates Lef1/TCF protein complex inhibiting the formation of the β-catenin Lef1/TCF DNA complex.
Full Name
Wingless-type MMTV Integration Site Family Member 5a
Source
E. coli
Species
Human
Accession #
P22725
Molecule Weight
The mature recombinant human Wnt-5a is a 38 kDa protein containing 343aa (Gln38-Lys380).
Purity
> 95 % as determined by SDS-PAGE
Endotoxin
< 0.1 EU per μg of the protein as determined by the LAL method
Activity
Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED50 for this effect is 0.1-0.5 µg/mL, in the presence of 5 ng/mL rmWnt-3a.
Storage
-80 Avoid repeated freeze-thaw cycles.
Storage Buffer
PBS
Additional information
OPTIONS | 50 ug (1 mg/ml), 1 mg (1 mg/ml), 100 mg (10 mg/ml), 1 g (10 mg/ml), Any size |
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