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MCLAB's All-In-One 5X Reverse Transcription Mix Kit is optimized to synthesize the first strand cDNA for RT-PCR and RT-qPCR.
First Strand cDNA Synthesis Kit can be used to synthesize the first strand cDNA.
SmartRTTM Reverse Transcriptase is an engineered MMLV RT that improves the thermostability of the enzyme, reduces RNase H activity and its cDNA synthesis ability. The enzyme also has a terminal transferase activity, where it adds a few extra nucleotides to the end of the synthesized cDNA. Together with a 3’ modified oligo dT primer and a 5’ SMART universal oligo, which contains a terminal complementation to nucleic acids at the 3’ end of the first-strand cDNA, the SmartRTTM reverse transcriptase produces RACE ready full-length cDNA.
Phage RNA polymerases are widely used for in vitro synthesis of RNA transcripts from DNA templates, which have a double-stranded promoter (at least 19 bases) upstream of the sequence to be transcribed.
The RNA Poly(A) Tailing Kit provides a highly pure and active enzyme and other reagents for quickly and easily adding a poly(A) tail to the 3' end of any RNA.
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