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Rnase III, <i>E. coli</i>
Rnase III, E. coli

Ribonuclease III (Rnase III) is an endoribunuclease that cleaves double-stranded RNA, resulting in 12-15 bp dsRNA fragments with 2-base, 3’-overhangs, and 5' phosphate and 3' hydroxyl termini. 
 
Name Cat# Size Concentration Price Qty
Rnase III, E. coli
RN3E-100
500 units
2,000 U/ml
$194.00
Rnase III, E. coli
RN3E-200
2,000 units
2,000 U/ml
$582.00
Rnase III, E. coli
RN3E-OEM
Any Size
please inquire
10X RNase III Reaction Buffer
B-R310
50 ml
$30.00

Description:
Ribonuclease III (Rnase III) is an endoribunuclease that cleaves double-stranded RNA, resulting in 12-15 bp dsRNA fragments with 2-base, 3’-overhangs, and 5' phosphate and 3' hydroxyl termini. 
 

Source:
Ribonuclease III (RNase III) from E. coli is an endoribonuclease that specifically digests dsRNA to dsRNA fragments that have two-base, 3' overhangs.

Applications:

  • Digestion of dsRNA to short fragments
  • RNA structure, processing, and maturation studies

Unit Definition:
1 unit of the Rnase III digests 1 µg of dsRNA in 20 minutes at 37°C

Supplied With:
10X RNase III Reaction Buffer

10X RNase III Reaction Buffer:
500 mM NaCl
100 mM Tris pH 7.9
100 mM MgCl2
10 mM DTT

Storage Buffer:
Supplied in 50% Glycerol, 50 mM Tris-HCl (pH 7.5),100 mM NaCl, 0.1 mM EDTA.

Recommended Storage Condition: -20°C

Manual & Protocols



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