|
Description:
SP Cross-linked Agarose Big Beads are strong ion exchangers designed for industrial applications. The large particle sizes (100-300 µm) and excellent physical stability of the base matrix ensures maintained speed, even with viscous samples. SP Cross-linked Agarose Big Beads are therefore the ultimate ion exchange media for initial purifications when high viscosity precludes the use of ion exchangers with smaller bead size, such as Cross-linked Agarose Fast Flow ion exchangers. The unique flow characteristics are also invaluable when adsorption needs to be done quickly.
Features:
-
Easy to scale-up
-
High flow rates
-
High chemical resistance for effective cleaning-in-place (CIP)
-
Easy maintenance
|
Product Specification
|
|
Ion exchanger type |
Sulfopropyl strong cation |
|
Ionic capacity |
0.18-0.25 mmol (H+)/ml |
|
Dynamic capacity |
70 mg RNase/ml medium |
|
Pressure/flow spec. |
1200-1800 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm |
|
Average particle size |
200 μm (100-300 μm) |
|
Matrix |
Highly cross-linked agarose, 6% |
|
pH stability |
3-14 (short term), 4-12 (long term) |
|
Chemical stability |
Stable in all common aqueous buffers:
8 M urea, 6 M guanidine HCl, 70% ethanol, 1 M NaOH*, and 1 M acetic acid*. |
|
Storage |
20% ethanol (Q, DEAE, ANX, CM), 0.2 M sodium acetate in 20% ethanol (SP) |
|
Storage temperature |
4°C to 30°C |
|
