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SUMO Protease
SUMO Protease

SUMO Protease has greater than 95% purity with no non-specific protease contamination. It recognizes the tertiary structure of SUMO and cleaves SUMO from recombinant fusion proteins.
 
Name Cat# Size Concentration Price Qty
SUMO Protease
SP-100
25,000 U
100 U/μl
$200.00
SUMO Protease
SP-200
100,000 U
100 U/μl
$600.00
SUMO Protease
SP-OEM
Any Size
please inquire

Description:
SUMO (Small Ubiquitin-like MOdifiers) Protease is a highly purified Ulp1 from a E. coli expression system, which carries the yeast Ulp1. It recognizes the tertiary structure of SUMO and cleaves SUMO from recombinant fusion proteins. The SUMO protease has a N-terminal His-tag and can be removed by Ni-NTA agarose.

Application:
- Removal of fusion tags from recombinant proteins
- Highly dynamic and precise cleavage capabilities
- Purification of proteins and peptides

Supplied with:
10X high salt and 10X no salt SUMO buffers

10× SUMO Protease Buffer (Salt plus)
500 mM Tris-HCl,pH 8.0
2% Igepal(NP-40)
1.5 M NaCl
10 mM DTT

10× SUMO Protease Buffer (Salt free)
500 mM Tris-HCl,pH 8.0
2% Igepal(NP-40)
10 mM DTT

SUMO Protease Storage Buffer
25 mM Tris-HCl,pH 8.0
0.1% Igepal(NP-40)
250 mM NaCl
0.5 mM DTT
50% glycerol 

Source: E. coli
M.W.: 26 kDa
Purity: ≥95%
Label: His-tag
Activity: >105 Unit/mg

Quality Control:
SUMO Protease has greater than 95% purity with no non-specific protease contamination. It is functionally tested for the absence of any non-specific protease activity.

Unit Definition:
One unit of SUMO Protease is defined as the amount of enzyme needed to cleave 85% of 2µg of substrate protein at 30°C in one hour.

Recommended Storage Condition: -80°C

Figures:
SUMO001.png
Figure 1, Coomassie staining of SUMO Protease in different elution fractions.
SUMO002.png
Figure 2, RP-HPLC Chromatogram of SUMO Protease.

Manual & Protocols



COA ( Certificate of Analysis )



MSDS & Certificates

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