
SmartRTTM Reverse Transcriptase is an engineered MMLV RT that improves the thermostability of the enzyme, reduces RNase H activity and its cDNA synthesis ability. The enzyme also has a terminal transferase activity, where it adds a few extra nucleotides to the end of the synthesized cDNA. Together with a 3’ modified oligo dT primer and a 5’ SMART universal oligo, which contains a terminal complementation to nucleic acids at the 3’ end of the first-strand cDNA, the SmartRTTM reverse transcriptase produces RACE ready full-length cDNA.
General Description SmartRTTM Reverse Transcriptase is an engineered MMLV RT that improves the thermostability of the enzyme, reduces RNase H activity and its cDNA synthesis ability. The enzyme also has a terminal transferase activity, where it adds a few extra nucleotides to the end of the synthesized cDNA. Together with a 3’ modified oligo dT primer and a 5’ SMART universal oligo, which contains a terminal complementation to nucleic acids at the 3’ end of the first-strand cDNA, the SmartRTTM reverse transcriptase produces RACE ready full-length cDNA.
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Manual & ProtocolsMSDS & Certificates |
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Lyse-load solution (5x1ml) | T4 Endonuclease VII | BL21(DE3)pLysS Competent E. coli |
$95.00
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$200.00
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$110.00
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HB101 Competent E. coli | Customized Competent E. coli | BL21(DE3) Competent E. coli |
$110.00
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$550.00
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$110.00
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T4 DNA Ligase | 2X HotStart PCR Master Mix | Phi29 DNA Polymerase |
$100.00
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$60.00
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$100.00
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