
Description:
Source: Specific Activity: 2000 U/µg Recommended Storage Condition: -20°C
Experimental Data:
Figure1. Performance of MCLAB’s T4 Endonuclease VII analyzed by capillary electrophoresis. (a) Negative control sample analysis, 10 pmol of FAM labeled 28mer oligonucleotide substrate. (b) 40 U of MCLAB’s T4 Endonuclease VII was able to fully resolve 10 pmol of FAM labeled 28mer oligonucleotide substrate with a 4-way Holliday junction structure (30 minutes at 37°C in 50 mM Tris-HCl, pH 8.0, 10 mM MgCl2, 10 mM 2-ME and 0.1 μg/μl BSA). For 4-way junction substrate: 1. Prepare the following reaction:
2. Mix well and incubate the reaction @ 37°C for 30min. 3. Use 1 µl reaction to analyze the cleaved fragments on Capillary electrophoresis.
For single base mismatch substrate: 1. Use about 200-400ng DNA fragment that contains single base mismatch for each reaction:
2. Mix well and incubate the reaction @ 37°C for 30min. 3. Run a 2% agarose gel and check for cleaved bands.
Table. Comparison of T4 Endonuclease VII and T7 Endonuclease I
* See Figure 2 for T4 endonuclease VII and T7 endonuclease I activity comparison result.
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