Description:
T7 DNA Ligase catalyzes the formation of a phosphodiester bond between a 5'-phosphate and a 3'-hydroxyl termini in duplex DNA. The enzyme joins blunt ends and cohesive ends termini as well as repair single-stranded nicks in duplex DNA.
Applications:
-
Joining of Okazaki fragments during replication
-
Completing short-patch DNA synthesis occurring in DNA repair process
Source:
A recombinant E. coli strain carrying the T7 DNA Ligase gene
Supplied in:
20 mM Tris-HCl
300 mM NaCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
pH 7.5 @ 25°C
10x Rapid Ligation Buffer:
660 mM Tris-HCl
100 mM MgCl2
10 mM DTT
10 mM ATP
75% PEG 6000
pH 7.6 @ 25°C
Unit Definition:
One Weiss unit of the enzyme catalyzes the conversion of 1 nmol of [32PPi] into Norit-adsorbable form in 20 min at 37°C. Enzyme activity is assayed in the following mixture: 66 mM Tris-HCl (pH 7.6), 6.6 mM MgCl2, 0.066 mM ATP, 10 mM DTT, 3.3 µM [32PPi].
One Weiss Unit is approximately equivalent to 67 cohesive end units (CEU). Or 1000 CEU = 15 Weiss Unit.
Specific Activity: 45 Weiss U/µg
Recommended Storage Condition: -20°C
Reference:
Doherty, A. et al. J.Biol. Chem (1996) V.271, No.19, 11083-11089.
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