T7 DNA Ligase

Description

Description:
T7 DNA Ligase catalyzes the formation of a phosphodiester bond between a 5′-phosphate and a 3′-hydroxyl termini in duplex DNA. The enzyme joins blunt ends and cohesive ends termini as well as repair single-stranded nicks in duplex DNA.

Applications:

• Joining of Okazaki fragments during replication
• Completing short-patch DNA synthesis occurring in DNA repair process

Source:
A recombinant E. coli strain carrying the T7 DNA Ligase gene

Supplied in:
20 mM Tris-HCl
300 mM NaCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
pH 7.5 @ 25°C

10x Rapid Ligation Buffer:
660 mM Tris-HCl
100 mM MgCl₂
10 mM DTT
10 mM ATP
75% PEG 6000
pH 7.6 @ 25°C

Unit Definition:
One Weiss unit of the enzyme catalyzes the conversion of 1 nmol of [³²PP_i] into Norit-adsorbable form in 20 min at 37°C.  Enzyme activity is assayed in the following mixture: 66 mM Tris-HCl (pH 7.6), 6.6 mM MgCl₂, 0.066 mM ATP, 10 mM DTT, 3.3 µM [³²PP_i].

One Weiss Unit is approximately equivalent to 67 cohesive end units (CEU). Or 1000 CEU = 15 Weiss Unit.

Specific Activity: 45 Weiss U/µg

Recommended Storage Condition: -20°C

Reference:
Doherty, A. et al. J.Biol. Chem (1996) V.271, No.19, 11083-11089.

Additional information

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