Description:
T7 DNA Polymerase is a mesophilic, highly processive, and replicative DNA polymerase from bacteriophage T7. It is responsible for the rapid and accurate replication of the virus genome during its infection cycle. T7 DNA Polymerase is a two subunit protein that consists of a polymerase domain (gene 5 from the T7 bacteriophage) and a processivity factor (E. coli trxA gene thioredoxin) (1, 2). The enzyme possesses a powerful (3' -> 5') nuclease activity that acts on both single and double stranded DNA and appears to be responsible for the high fidelity of this enzyme and prevents strand displacement synthesis(3,4,5).
Specific Activity: 10,000U/mg
Application:
Second strand synthesis in site-directed mutagenesis protocols
Source:
A recombinant E. coli strain carrying the bacteriophage T7 gene 5.
Unit Definition:
1 unit is defined as the amount of polymerase required to convert 10 nmol of total dNTPs into acid insoluble material in 30 minutes at 37°C.
Supplied in:
50 mM KPO4
0.1 mM EDTA
1.0 mM Dithiothreitol
50% Glycerol
pH 7.0 @ 25°C
Supplied with:
10x T7 DNA Polymerase Buffer
10x T7 DNA Polymerase Buffer:
400 mM Tris-HCl
200 mM MgCl2
500 mM NaCl
pH 7.5 @ 25°C
Recommended Storage Condition: -20°C
Reference:
1. Grippo, P. et al. (1971) J. Biol. Chem. 246, 6867-6873
2. Modrich, P. et al. (1975) J. Biol. Chem. 250, 5515-5522
3. Adler, S. et al. (1979) J. Biol. Chem. 254, 11605-11614
4. Hori, K., et al. (1979) J. Biol. Chem. 254, 11598-11604
5. Lechner, R. L. et al. (1983) J. Biol. Chem. 258, 11185-11196
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