T7 Exonuclease

T7 Exonuclease
Description

T7 Exonuclease is similar to Lambda Exonuclease in that it catalyzes the stepwise hydrolysis of duplex DNA from the 5' termini liberating 5' mononucleotides. However, unlike Lambda Exonuclease, the enzyme has low processivity and it will remove both 5-hydroxyl and 5-phosphcryl termini.
 
Name Cat# Size Concentration Price Qty
T7 Exonuclease
T7G6E-100
5,000 units
10,000 U/ml
$115.00
T7 Exonuclease
T7G6E-200
20,000 units
10,000 U/ml
$180.00
T7 Exonuclease
T7G6E-300
100,000 units
10,000 U/ml
$360.00
T7 Exonuclease
T7G6E-OEM
Any Size
please inquire
Description:
T7 Exonuclease is similar to Lambda Exonuclease in that it catalyzes the stepwise hydrolysis of duplex DNA from the 5'-termini, liberating 5'-mononucleotides. However, unlike Lambda Exonuclease, the enzyme has low processivity and it will remove both 5'-hydroxyl and 5'-phosphoryl termini. T7 Exonuclease hydrolyzes duplex DNA non-processively in the 5' -> 3' direction from both 5'-phosphoryl or 5'-hydroxyl nucleotides by liberating oligonucleotides, as well as mononucleotides, until about 50% of the DNA is acid soluble.

Source:
Purified from an E. coli strain containing a TYB12 intein fusion.

Storage Conditions
10 mM Tris-HCl
5 mM DTT
0.1 mM EDTA
50% Glycerol
pH 8.0 @ 25°C

Application:
- Controlled stepwise digestion of double-stranded DNA from the 5'-termini.
- Generating ssDNA templates for sequencing via the chain-termination method.

Supplied with:
10X T7 Exonuclease Buffer

10X T7 Exonuclease Buffer:
500 mM Potassium Acetate
200 mM Tris-acetate
100 mM Magnesium Acetate
10 mM DTT
pH 7.9 @ 25°C

Unit Definition:
One unit is the amount of enzyme required to release 1 nmol of acid soluble nucleotide in 15 min at 37°C under standard assay conditions.

Recommended Storage Conditions: -20°C

Reference:
1. Kerr, C. and Sadowski, P. D. (1972) J. Biol. Chem. 247, 311-318.
2. Thomas, K. R. and Olivera, B. M. (1978) J. Biol. Chem. 253, 424-429.
3. Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A. and Struhl, K., (1987) Current Protocols in Molecular Biology (John Wiley and Sons, Inc.
4. Shon, M., Germino, J. and Bastia, D. (1982) J. Biol. Chem. 257, 13823-13827.
5. Nikiforov, T. T., Rendle, R. B., Goelet, P., Rogers, Y. H., Kotewicz,
6. M. L., Anderson, S., Trainor, G. L. and Knapp, M. R. (1994) Nucl. Acids Res 22, (20), 4167-4175.
7. Kornberg, A. and Baker, T. (1991) DNA Replication, Second Edition, 591.

Manual & Protocols



COA ( Certificate of Analysis )



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