T7 Exonuclease
T7 Exonuclease

T7 Exonuclease is similar to Lambda Exonuclease in that it catalyzes the stepwise hydrolysis of duplex DNA from the 5' termini liberating 5' mononucleotides. However, unlike Lambda Exonuclease, the enzyme has low processivity and it will remove both 5’-hydroxyl and 5’-phosphoryl termini.
 
Name Cat# Size Concentration Price Qty
T7 Exonuclease
T7G6E-100
2,500 units
10,000 U/ml
$102.00
T7 Exonuclease
T7G6E-200
10,000 units
10,000 U/ml
$306.00
T7 Exonuclease
T7G6E-OEM
Any Size
please inquire
10X T7 Exonuclease Buffer
B-T7E10
50 ml
$40.00

Description:
T7 Exonuclease is similar to Lambda Exonuclease in that it catalyzes the stepwise hydrolysis of duplex DNA from the 5'-termini, liberating 5'-mononucleotides. However, unlike Lambda Exonuclease, the enzyme has low processivity and it will remove both 5'-hydroxyl and 5'-phosphoryl termini. T7 Exonuclease hydrolyzes duplex DNA non-processively in the 5' -> 3' direction from both 5'-phosphoryl or 5'-hydroxyl nucleotides by liberating oligonucleotides, as well as mononucleotides, until about 50% of the DNA is acid soluble.

Source:
Purified from an E. coli strain containing a TYB12 intein fusion.

Storage Conditions
10 mM Tris-HCl
5 mM DTT
0.1 mM EDTA
50% Glycerol
pH 8.0 @ 25°C

Application:
- Controlled stepwise digestion of double-stranded DNA from the 5'-termini.
- Generating ssDNA templates for sequencing via the chain-termination method.

Supplied with:
10X T7 Exonuclease Buffer

10X T7 Exonuclease Buffer:
500 mM Potassium Acetate
200 mM Tris-acetate
100 mM Magnesium Acetate
10 mM DTT
pH 7.9 @ 25°C

Unit Definition:
One unit is the amount of enzyme required to release 1 nmol of acid soluble nucleotide in 15 min at 37°C under standard assay conditions.

Recommended Storage Condition: -20°C

Reference:
1. Kerr, C. and Sadowski, P. D. (1972) J. Biol. Chem. 247, 311-318.
2. Thomas, K. R. and Olivera, B. M. (1978) J. Biol. Chem. 253, 424-429.
3. Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A. and Struhl, K., (1987) Current Protocols in Molecular Biology (John Wiley and Sons, Inc.
4. Shon, M., Germino, J. and Bastia, D. (1982) J. Biol. Chem. 257, 13823-13827.
5. Nikiforov, T. T., Rendle, R. B., Goelet, P., Rogers, Y. H., Kotewicz,
6. M. L., Anderson, S., Trainor, G. L. and Knapp, M. R. (1994) Nucl. Acids Res 22, (20), 4167-4175.
7. Kornberg, A. and Baker, T. (1991) DNA Replication, Second Edition, 591.

Manual & Protocols



COA ( Certificate of Analysis )



MSDS & Certificates

There is no supporting data for this product. Will be available soon!!
McBead™-50 Magnetic Stand
McBead™-96 Magnet Plate
McBead™ -6 Magnet Stand
McBead™-50 Magnetic Stand McBead™-96 Magnet Plate McBead™ -6 Magnet Stand
$300.00
$500.00
$200.00
 
Specialized DNA Sequencing Services
I-5™ Hi-Fi DNA Polymerase
SmartRT™ Reverse Transcriptase Kit
Specialized DNA Sequencing Services I-5™ Hi-Fi DNA Polymerase SmartRT™ Reverse Transcriptase Kit
$1.99
$50.00
$50.00
 
I-5™ Hi-Fi HotStart DNA Polymerase
All-In-One 5X Reverse Transcription Mix Kit
Whole Genome Sequencing
I-5™ Hi-Fi HotStart DNA Polymerase All-In-One 5X Reverse Transcription Mix Kit Whole Genome Sequencing
$50.00
$100.00
$1000.00