
Phage RNA polymerases are widely used for in vitro synthesis of RNA transcripts from DNA templates, which have a double-stranded promoter (at least 19 bases) upstream of the sequence to be transcribed.
Description:
Components The kit contains sufficient materials for 20 or 100 reactions at 20 μL total reaction volume. 20 rxns Components 20 μL T7 Enzyme Mix 20 μL ATP Solution (50 mM) 20 μL CTP Solution (50 mM) 20 μL GTP Solution (50 mM) 20 μL UTP Solution (50 mM) 20 μL 100 mM DTT 250 μL 10X Transcription Buffer 10 μL Control DNA Template (0.5 μg/μL) 1.5 mL Nuclease-free Water 100 rxns Components 100 μL T7 Enzyme Mix 100 μL ATP Solution (50 mM) 100 μL CTP Solution (50 mM) 100 μL GTP Solution (50 mM) 100 μL UTP Solution (50 mM) 100 μL 100 mM DTT 250 μL 10X Transcription Buffer 10 μL Control DNA Template (0.5 μg/μL) 1.5 mL Nuclease-free Water
Recommended Storage Condition: -20°C Shipping Condition: Dry ice
Reference: |
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