New special offers are available!
TEV Protease
TEV Protease

TEV (Tobacco etch virus) Protease is a highly site-specific cysteine protease that recognizes the cleavage site of Glu-Asn-Leu-Tyr-Phe-Gln-Gly and cleaves between Gln and Gly. TEV protease is a very useful reagent for cleaving fusion proteins due to its high specificity and high activity rate.

 
Name Cat# Size Concentration Price Qty
TEV Protease
TEP-100
1 mg
1mg/ml
$175.00
TEV Protease
TEP-200
4 mg
1mg/ml
$525.00
TEV Protease
TEP-OEM
Any Size
please inquire

Description:
TEV (Tobacco Etch Virus) Protease is a highly site-specific cysteine protease that recognizes the cleavage site of Glu-Asn-Leu-Tyr-Phe-Gln-Gly and cleaves between Gln and Gly. TEV protease is a very useful enzyme for cleaving fusion proteins due to its high specificity and its high activity rate. TEV Protease contains a N-terminal His-tag, and it can be removed by Ni-NTA column after cleavage reaction.

Applications:

  • Removal of fusion tags from recombinant proteins
  • Highly dynamic and precise cleavage capabilities
  • purification of proteins and peptides


Supplied With:
TEV substrate, 50 µl
10X TEV buffer

Source:
E. coli derived from Tobacco Etch Virus.

Label: His-tag

Reaction Conditions:
TEV protease is maximally active at 34°C, but its recommended to perform digests at room temperature (20°C) or 4°C. The activity of TEV protease is approximately 3-fold greater at 20°C than at 4°C.

10X TEV buffer
500 mM Tris-HCl, 1.5 M NaCl, pH 8.0

Recommended Storage Condition: -20°C

Figures:
turbotev01.png
Figure 1
turbotev02.png
Figure 2, 69.8 kDa TEV substrate protein is incubated with TurboTEV. The cleaved products are 41.2 kDa and 27.7 kDa, respectively.

Manual & Protocols



MSDS & Certificates

The Q&A for this product will be available soon.
Small RNAs Cloning
DNA Size Standard
<i>E. coli.</i> (DH5a) Cell Lysate
Small RNAs Cloning DNA Size Standard E. coli. (DH5a) Cell Lysate
$238.00
$105.00
 
BDX64 (BigDye<sup></sup> Enhancing Buffer)
Primers for Sequencing
Human Genomic DNA
BDX64 (BigDye Enhancing Buffer) Primers for Sequencing Human Genomic DNA
$210.00
$45.00
$72.00
 
2X  HoTaq&trade;  qPCR Kit For TaqMan<sup>&reg;</sup>
DNA Mutagenesis Services
DNA Polymerase I
2X HoTaq™ qPCR Kit For TaqMan® DNA Mutagenesis Services DNA Polymerase I
$266.00
$100.00