Thermostable RNase H
Thermostable RNase H

Thermostable RNase H degrades the RNA strand in RNA-DNA hybrids, it has an optimal activity above 65°C and can be used up to 95°C.
 
Name Cat# Size Concentration Price Qty
Thermostable RNaseH
HTRH-100
1,250 units
5 U/µl
$363.69
Thermostable RNaseH
HTRH-200
5,000 units
5 U/µl
$566.50
Thermostable RNaseH
HTRH-OEM
Any Size
please inquire

Description:
Thermostable RNase H has an optimal activity above 65°C and can be used up to 95°C. The enzyme degrades RNA in a DNA:RNA hybrid, maximizing sensitivity and selectivity without affecting DNA or unhybridized RNA.

Applications:

  • High hybridization stringency
  • Specific hydrolysis of RNA in a DNA:RNA hybrid
  • Diagnostic assay of DNA sequences by isothermal probe amplification
  • Mapping of mRNA structures

Source:
A recombinant protein purified from E. coli, cloned the gene encoding the Thermus thermophilus RNase H

Unit Definition:
One unit of the enzyme results in the acid-solubilization of 1 nmol of polyadenylic acid in the presence of an equimolar concentration of polythymidylic acid in 20 minutes at 45°C in 50 mM Tris-HCl(pH 7.5), 100 mM NaCl, and 10 mM MgCl2. Note: The unit assay is performed at 45°C because this is optimal for the Tm of poly(dT):poly(A). The optimal temperature for many applications may be considerably higher.


10x RNAse H Buffer:
500 mM Tris-HCl
750 mM KCl
30 mM MgCl2
100 mM DTT
pH 8.3 @ 25°C


Storage Buffer:
Supplied in 50% Glycerol containing 50 mM Tris-HCl (pH 7.5), 0.1 M NaCl, 1.0 mM DTT, 0.1 mM EDTA, and 0.1% Triton X-100.

Recommended Storage Condition: -20°C

Manual & Protocols



COA ( Certificate of Analysis )



MSDS & Certificates

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