Description:
MCLAB's HRC3C Protease is fused with both GST and His tags, so that the protease is easily removed form cleavage reactions by either immobilized glutathione (GSH) resin or immobilized Ni-chelating resin. Turbo HRV3C Protease is highly active at 4ºC, and also active in a variety of commonly used buffers, providing more flexibility in experimental design to keep the activity and intact structure of the target proteins.

Application:
MCLAB's Turbo Human Rhinovirus (HRV) 3C Protease is a recombinant cysteine protease used to remove fusion tags from proteins with the HRV3C cleavage sequence. HRC3C Protease is highly specific for the PreScission Site, Leu-Glu-Val-Leu-Phe-Gln-↓-Gly-Pro, and cleaves between the Gln and Gly residues.

Supplied With:
HRV 3C Substrate, 100 µl (1 mg/ml)
10x HRV3C Protease Reaction buffer (10 ml)

HRV3C Protease 10X Cleaveage Buffer:
500 mM Tris-HCl (pH 8.0)
1.5 M NaCl

Figure:
Figure 1. SDS-PAGE Analysis of Control Protein by Turbo HRV3C Protease
A 70 kD GST-fusion protein (1) at 1 mg/ml is reacted with Turbo HRV3C Protease at a ratio of 1:100 (w/w) in cleavage buffer at 4ºC for 4 hour (2) 3 days (3) The cleaved products are 45 kD and 25 kD.

Source: E. coli

Supplied In:
50 mM Tris-HCl (pH 8.0)
150 mM NaCl
1 mM EDTA
1 mM DTT
20% glycerol

Unit definition:
1 unit will cleave >95% of 100 µg His-tag fusion control protein in 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 at 4°C for 16 h.

Recommended Storage Condition: -20°C