Description:
TurboTEV (Tobacco Etch Virus) Protease is a highly enhanced site-specific cysteine protease that recognizes the cleavage site of Glu-Asn-Leu-Tyr-Phe-Gln-Gly and cleaves between the Gln and Gly. TurboTEV protease is resistant to auto-inactivation under normal reaction conditions and works as a better catalyst than the wild-type enzyme. It is a very useful enzyme for cleaving fusion proteins due to its high specificity and high activity rate without the requirements of specialized buffer. It has both a His-tag and GST tag, which allow it to be removed by Ni-chelating or GSH resin.

Applications:

• Removal of fusion tags from recombinant proteins
• Highly dynamic and precise cleavage capabilities
• Purification of proteins and peptides

Source:
E. coli derived from Tobacco Etch Virus.

Supplied With:
TEV substrate, 50 µl
10X TEV buffer

Label: His-tag and GST tag

Reaction Conditions:
Turbo TEV protease is maximally active at 34°C, but its recommended to perform digests at room temperature (20°C) or 4°C. The activity of TEV protease is approximately 3-fold greater at 20 °C than at 4°C.

10X TEV buffer
500 mM Tris-HCl, 1.5 M NaCl, pH 8.0

Recommended Storage Condition: -20°C

Figures:

Figure 1

Figure 2. 69.8 kDa TEV substrate protein is incubated with TurboTEV. The cleaved products are 41.2 kDa and 27.7 kDa, respectively.