Description
Cas13d is from Ruminococcus flavefaciens(casRX) and it has 94KD. The dcas13d has mutants which can not cut the target RNA but binds.

Applications
• Targeted RNA knockdown
• RNA editing
• Nucleic acid detection

Source
E. coli

Organism
Leptotrichia wadeii (strain F0279)

UniProt #
U2PSH1

Molecule Weight
The mature recombinant Cas13a is a 143.7 kDa protein containing 1182aa.

Purity
> 95 % as determined by SDS-PAGE

Storage
-20 Avoid repeated freeze-thaw cycles.

Cas13 Storage Buffer
0.3 M NaCl
20 mM Tris-HCl, pH 8.0
0.5 mM EDTA
1 mM DTT
50% Glycerol

10X Cas13 Reaction Buffer
200 mM HEPES, 50 mM MgCl₂, 1 M NaCl, 1 mM EDTA, pH 6.5

10X Cas13 sherlock reaction buffer (requested)
200 mM HEPES, 90 mM MgCl₂, pH 6.8