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2X Ori-Master Mix

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BrightDye Terminator Cycle Sequencing Kit

The 2X Ori-Master Plasmid Amplification Mix is a premixed, ready-to-use solution containing a high-fidelity DNA polymerase, ori specific primers, dNTPs, and MgCl2. The Master Mix can produce linear copies of bacterial plasmids from a single colony. The Master Mix is ideal for fast, high throughput colony PCR, bacterial screening, and sequencing.

  Product Highlights:
  • Fast reaction : less than 2hrs from a colony to its DNA products, comparing to 16-24 hours for a miniprep process
  • Good yield: a 25 µl reaction yields up to µg DNA
  • Easy to use: Ori-specific primers are included and the PCR protocol is optimized. Just add the template and water.
  • Long amplification fragments: up to 10 Kb
  • High throughput capability: 96 or 384 well plate compatible
  Experimental Data:     Applications:  
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  • Colony PCR
  • DNA Sequencing
  • Restriction Digestion
  Click Here To Order : $1/Reaction  


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qPCR Library Quantification

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