EDTA, 0.5 M, pH 8.0, is a sterile-filtered solution of 0.5 M EDTA in H2O treated with diethyl pyrocarbonate (DEPC). EDTA (Ethylenediaminetetraacetic acid) is a chelating agent, a general chemical, and a sequestrant. Suitable for use in molecular biology applications. EDTA is used extensively as chelator of divalent cations such as Ca2+. Useful in molecular biology applications to minimize metal ion contaminants. Also used to facilitate enzymatic reactions that could be inhibited by heavy metal traces. Inhibits enzymes, such as metalloproteases, that require divalent cations for activity.
Phosphate-buffered saline (PBS) is a balanced salt solution that is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. PBS is formulated without calcium and magnesium for rinsing chelators from the culture before cell dissociation.
Magnesium is a cofactor for thermostable DNA polymerases. This patent-pending technology relates to thermally reversible chelators of magnesium, thus a hot-start-cold-stop PCR is achieved.
The MCLAB's HoTaq Taqman probe One-step Real-time RT-PCR Kit offers a unique system for performing probe based realtime RT-PCR in a single step within a single tube with an optimized reaction condition, which utilizes our own proprietary engineered QuantumScript HD reverse transcriptase and hot-start Taq DNA Polymerase with high purity . No additional reagents or steps are required once the reaction is initialized. This novel kit enables to quantitatively detect specific RNA targets with high sensitivity, unparalleled convenience, and a wide dynamic range.
2x BlueStarTM PCR Master Mix contains all the necessary reagents and a gel loading dye. The PCR mix can be directly loaded onto a DNA gel.
The MCLAB's HoTaq™ SYBR Green One-Step RT-PCR Kit allows researchers to perform SYBR Green based RT-qPCR in a single step within a single tube. The kit utilizes our own proprietary engineered QuantumScript HD reverse transcriptase and hot-start Taq DNA polymerase. No additional reagents or steps are required once the reaction is initiated. The kit enables the quantification of specific RNA targets with high sensitivity, unparalleled convenience, and wide dynamic range.
Friendly Reminder : This product is a replacement of an equivalent product : 2X Universal Taq Master Mix.
The 2X Ori-Master Mix is a premixed, ready-to-use solution containing a high-fidelity DNA polymerase, ori specific primers, dNTPs, and MgCl2.
The 2X STR Master Mix allows for even and reliable multiplex PCR analysis of microsatellite loci.
