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T3 DNA Ligase
T3 DNA Ligase catalyzes the formation of a phosphodiester bond between a 5′ phosphate and a 3′ hydroxyl terminus in duplex DNA. The enzyme joins blunt ends and cohesive ends as well as repair single stranded nicks in duplex DNA.
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T4 DNA Helicase
T4 replication helicase (gp41) and polymerase (gp43) can be assembled onto a loading site located near the end of a long double-stranded DNA template in the presence of a macro-molecular crowding agent, and that this coupled “two-protein” system can carry out ATP-dependent strand displacement DNA synthesis at physiological rates (400 to 500 bp per sec) and with high processivity in the absence of other T4 DNA replication proteins.
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T4 DNA Ligase
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA using ATP as a cofactor.
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T4 DNA Polymerase
T4 DNA Polymerase catalyzes the extension of a primed DNA template in the 5′→ 3′ direction. This enzyme exhibits a powerful 3′→ 5′ exonuclease activity; it lacks any inherent 5′→ 3′ exonuclease or strand displacement functions.
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T4 Endonuclease VII
Endonuclease VII is the product of gene 49 of bacteriophage T4. It has a mass of 18 kDa. T4 Endonuclease VII involves in DNA-packaging, genetic recombination and mismatch repair in vivo.
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T4 Lysozyme
Bacteriophage T4 Lysozyme breaks down bacterial cell walls. The enzyme attacks the peptidoglycans in the cell walls of bacteria and hydrolyzes the β-1,4 linkages between N-acetylmuramic acid and N-acetylglucosamine. Specific activity of T4 lysozyme is significantly greater than egg white lysozyme when assayed with Micrococcus lysodeikticus and E. coli.
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T4 Polynucleotide Kinase
T4 Polynucleotide Kinase (PNK) catalyzes the transfer and exchange of the terminal gamma phosphate of ATP to the 5′-hydroxyl terminus of double-and single-stranded DNA, RNA and nucleoside 3′-monophosphate molecules (1).
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T4 RNA Ligase 1 (ssRNA Ligase)
T4 RNA Ligase 1 catalyzes the ligation of a 5' phosphoryl-terminated nucleic acid donor to a 3' hydroxyl-terminated nucleic acid acceptor through the formation of a 3'→5' phosphodiester bond, with hydrolysis of ATP to AMP and PPi. Substrates include single-stranded RNA and DNA as well as dinucleoside pyrophosphates.
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T4 RNA Ligase 2 (dsRNA Ligase)
T4 RNA Ligase 2, also known as T4 Rnl2 (gp24.1), has both intermolecular and intramolecular RNA strand joining activity. Unlike T4 RNA Ligase 1, T4 RNA Ligase 2 is much more active joining nicks on double stranded RNA than on joining the ends of single stranded RNA. The enzyme requires an adjacent 5´ phosphate and 3´ OH for ligation.
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T4 RNA Ligase 2 (truncated) (RNL2)
MCLAB’s truncated  T4 RNA Ligase 2 was developed specifically for demanding Next-Generation RNA Sequencing applications.  The truncated  ligase 2 specifically ligates the adenylated 5´ end of an adapter to the 3´ end of RNA. The enzyme does not require ATP for ligation but does need an adenylated substrate. By not having an extra ATP in the reaction it dramatically reduces the amount of ligation between random RNA molecules.
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T7 DNA Ligase
T7 DNA Ligase catalyzes the formation of a phosphodiester bond between a 5′ phosphate and a 3′ hydroxyl termini in duplex DNA. The enzyme joins blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA.
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T7 DNA Polymerase
T7 DNA Polymerase is a mesophilic, highly processive, and replicative DNA polymerase from bacteriophage T7. It is responsible for the rapid and accurate replication of the virus' genome during its infection cycle.
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T7 Exonuclease
T7 Exonuclease is similar to Lambda Exonuclease in that it catalyzes the stepwise hydrolysis of duplex DNA from the 5' termini liberating 5' mononucleotides. However, unlike Lambda Exonuclease, the enzyme has low processivity and it removes both 5’-hydroxyl and 5’-phosphoryl termini.
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T7 High Yield In Vitro Transcription Kit

Phage RNA polymerases are widely used for in vitro synthesis of RNA transcripts from DNA templates, which have a double-stranded promoter (at least 19 bases) upstream of the sequence to be transcribed.

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T7 RNA Polymerase
T7 RNA polymerase is a DNA-dependent RNA polymerase that catalyzes the formation of RNA in the 5′→ 3′ direction.
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TAE (Tris/Acetate/EDTA) Buffer, 10X

TAE buffer is the most commonly used buffer for DNA agarose gel electrophoresis but is also used for non-denaturing RNA agarose gel electrophoresis1. Double-stranded DNA tends to run faster in TAE than in other buffers but can also become exhausted during extended electrophoresis. Dilution of the concentrated TAE buffer produces a 1× TAE buffer with 40 mM Tris-acetate and 1 mM EDTA, pH 8.3. The 1× TAE buffer is used both in the agarose gel and as a running buffer.

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Terminal deoxynucleotidyl Transferase (TdT)
Terminal deoxynucleotidyl transferase (TdT) is a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3’ hydroxyl terminus of single or double stranded DNA molecules.
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Terrific Broth Agar Plates
1.2% Bacto tryptone, 2.4% yeast extract, 1.6% glycerol, 1.5% agar, 72mM K2HPO4, and 17mM KH2PO4.
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TEV Protease

TEV (Tobacco etch virus) Protease is a highly site-specific cysteine protease that recognizes the cleavage site of Glu-Asn-Leu-Tyr-Phe-Gln-Gly and cleaves between Gln and Gly. TEV protease is a very useful reagent for cleaving fusion proteins due to its high specificity and high activity rate.

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